Cycloheximide

Catalog No.S7418 Batch:S741804

Technical Data

Formula	C₁₅H₂₃NO₄
Molecular Weight	281.35	CAS No.	66-81-9
Solubility (25°C)*	In vitro	DMSO	56 mg/mL (199.04 mM)
		Ethanol	56 mg/mL (199.04 mM)
		Water	15 mg/mL (53.31 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Cycloheximide, an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy.Solutions are unstable and should be fresh-prepared.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
In vitro	Cyclohexamide, a protein synthesis inhibitor, reduces GSIV-induced host cell death by attenuating phosphatidylserine exposure and loss of ΔΨm from apoptosis/necrosis.^[1]
In vivo	Cycloheximide, a protein synthesis inhibitors, acts on memory by altering modulators of memory formation as a secondary consequence of the inhibition of protein synthesis.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines grouper fin cell line (GF-1) Concentrations 0.33 µg/ml Incubation Time 0-5 days Method Monolayers of GF-1 cells (4.0 mL, 10⁵ cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.
Animal Study:^{^[2]}	Animal Models Male ICR mice Dosages 30mg/kg, 60mg/kg,120 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
grouper fin cell line (GF-1)
Concentrations
0.33 µg/ml
Incubation Time
0-5 days
Method
Monolayers of GF-1 cells (4.0 mL, 10⁵ cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.

Animal Study:^{^[2]}

Animal Models
Male ICR mice
Dosages
30mg/kg, 60mg/kg,120 mg/kg
Administration
i.p.

References

[4] Santos DA, et al. Nucleic Acids Res. 2019;47(10):4974-4985.

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Selleck's Cycloheximide has been cited by 431 publications

Effective targeting of PDGFRA-altered high-grade glioma with avapritinib [ Cancer Cell, 2025, S1535-6108(25)00070-4]	PubMed: 40086436
VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26]	PubMed: 39848960
The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis [ Nat Commun, 2025, 16(1):2039]	PubMed: 40016204
ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylation [ Nat Commun, 2025, 16(1):2071]	PubMed: 40021646
The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism [ Mol Cell, 2025, 85(3):638-651.e9]	PubMed: 39889707
OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model [ Adv Sci (Weinh), 2025, 12(7):e2406700]	PubMed: 39721018
Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1 [ Theranostics, 2025, 15(7):3207-3222]	PubMed: 40083918
Glutathione reductase underlies the stability of mutant p53 by antagonizing protein glutathionylation [ Redox Biol, 2025, 81:103522]	PubMed: 39983342
Circ-0001283 Aggravates Cardiac Hypertrophy by Targeting Myosin Light Chain 3 Protein [ Research (Wash D C), 2025, 8:0626]	PubMed: 40007621
Hypoxia-induced degradation of FTO promotes apoptosis by unmasking RACK1-mediated activation of MTK1-JNK1/2 pathway [ J Adv Res, 2025, S2090-1232(25)00038-4]	PubMed: 39805423

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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